CRISPR-assisted test for Schistosoma haematobium

CRISPR-assisted test for Schistosoma haematobium

Blog Article

Abstract Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings.Here Pre-Rinse Faucets we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection.CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species.Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2 h.The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries.

This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in LIVER PLUS remote areas and could have a significant impact on the elimination of neglected tropical diseases.